High-Throughput Craniofacial Development Phenotyping in Zebrafish using VAST BioImager
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چکیده
Zebrafish is an emerging vertebrate model organism to study human diseases and toxicology [1]. A variety of genetic tools in zebrafish allow in vivo modeling of human genetic diseases and regeneration [2]. Zebrafish larvae also have small size and optical clarity which allows easy microscopic evaluation of physiological changes and morphological phenotypes. Conventional imaging approaches involve manual orientation of each larva and are difficult to automate for genetic and drug screens. Here we report how VAST BioImager [3] was used to automate zebrafish larvae positioning, orientation and image collection for a large numbers of zebrafish larvae. Mutations in mapre2 gene lead to defects in normal craniofacial development in zebrafish larvae [4]. Mutations in human MAPRE2 are linked to circumferential skin creases Kunze type (“Michelin tire baby”) [5]. MAPRE2 encodes a member of tubulin binding family and is thought to control microtubule dynamics and reorganization [6].
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